嘌呤霉素 Puromycin 10 mg/mL
Certificates of Analysis
Cross Reference Table
Catalog Number:S250J0
Size:1 mL
Product Description
嘌呤霉素(Puromycin)是由白黑链霉菌(Streptomyces alboniger)发酵代谢产生的一种氨基糖苷类抗生素,通过抑制蛋白质合成而杀死革兰氏阳性菌及各种动物和昆虫细胞,某种特殊情况下也有效作用于大肠杆菌。嘌呤霉素作为氨酰-tRNA分子3’末端的类似物,能够与核糖体的A位点结合并掺入到延伸的肽链中。但是嘌呤霉素同A位点结合后,不会参与随后的任何反应,从而导致蛋白质合成的提前终止并释放出C-末端含有嘌呤霉素的不成熟多肽。
对嘌呤霉素的抗性取决于编码嘌呤霉素N-乙酰转移酶(PAC)的pac基因,遗传工程研究使用的pac基因分离自嘌呤霉素产生菌Streptomyces alboniger。
嘌呤霉素普遍应用于筛选和维持培养含pac基因的哺乳动物稳定转染细胞。嘌呤霉素在细胞稳转株筛选中的普遍应用与慢病毒载体的特性有关,现在商业化的慢病毒载体多数都携带pac基因。在某些特定情况下,嘌呤霉素亦可以用来筛选转化携带pac基因质粒的大肠杆菌菌株。
本产品使用注射用水(Water-For-Injection)配制。
本产品供科学研究使用,用于组织和细胞的体外培养。禁止临床使用。
Quality Assurance
Products from Shanghai BasalMedia Technologies Co.,LTD are all manufactured in cGMP-compliant facilities. Shanghai BasalMedia Technologies Co.,LTD is certified with ISO9001:2015 and ISO13485:2016 quality system standards.
Manufacturing Assurance
We have been committed to adopting modern management models to make all production and operation activities, including safety production management, scientific, standardized and legalized. It comprehensively promotes the occupational health and safety management system in a scientific way to create a healthy, safe and friendly working environment for every employee of BasalMedia Technologies. We have passed the ISO45001:2018 Occupational Health and Safety Management System Certification and ISO14001:2015 Environmental Management System Certification.
Catalog Number |
Size |
Price (CNY) |
Availability |
S250J0 |
1 mL |
400.00 |
|
Specifications
Application Area |
细胞培养相关试剂 |
Product Category |
细胞培养抗生素 |
Form |
液体 |
Classification |
无动物源 |
Sterility |
过滤除菌 |
Shelf Life |
24个月 |
With/Without Additives(+) |
[+]10 mg/mL 嘌呤霉素 |
Endotoxin |
≤5 EU/mL |
pH Level |
6.0~7.0 |
Storage Conditions |
-30~-5 ℃,避光 |
Shipping Conditions |
干冰 |
Application Area |
细胞培养相关试剂 |
Product Category |
细胞培养抗生素 |
Form |
液体 |
Sterility |
过滤除菌 |
Shelf Life |
24个月 |
With/Without Additives(+) |
[+]10 mg/mL 嘌呤霉素 |
Endotoxin |
≤5 EU/mL |
pH Level |
6.0~7.0 |
Storage Conditions |
-30~-5 ℃,避光 |
Classification |
无动物源 |
Shipping Conditions |
干冰 |
Documents
Certificates of Analysis
Cross Reference Table
Quality Assurance
Products from Shanghai BasalMedia Technologies Co.,LTD are all manufactured in cGMP-compliant facilities. Shanghai BasalMedia Technologies Co.,LTD is certified with ISO9001:2015 and ISO13485:2016 quality system standards.
Manufacturing Assurance
We have been committed to adopting modern management models to make all production and operation activities, including safety production management, scientific, standardized and legalized. It comprehensively promotes the occupational health and safety management system in a scientific way to create a healthy, safe and friendly working environment for every employee of BasalMedia Technologies. We have passed the ISO45001:2018 Occupational Health and Safety Management System Certification and ISO14001:2015 Environmental Management System Certification.